文章基本信息

标题：フォスファターゼの簡易試験法
本地全文：下载
作者：平山清 ; 岡本京子
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1969
卷号：15
期号：1
页码：37-39
DOI：10.1248/jhs1956.15.37
出版社：The Pharmaceutical Society of Japan
摘要：The Japanese official method for alkaline phosphatase in milk is Sanders-Seger's method which is so complicated that it can be hardly practised in daily routine. Accordingly, milk technicians hope to have a more simple procedure. The method using p-nitrophenyl phosphate as a substrate was ascertained to be simple and practicable. By means of this method, the presence of raw milk can be easily detected, even when it was diluted 1000-2000 times with pure water. The reagent is composed of 100 ml of buffer solution (Na OAc, NaCl, MgSO₄ and barbital Na) and 1.5 mg of sodium p-nitrophenyl phosphate. To discriminate yellow coloration due to the presence of alkaline phosphatase, 1 ml of this reagent is added to 1 ml of the sample for testing.