文章基本信息

标题：グリオキザルビス(2-ヒドロキシアニル)による微量血清中のカルシウムの比色定量法について
本地全文：下载
作者：中隈雅敬 ; 扇谷正三郎
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1969
卷号：15
期号：4
页码：232-237
DOI：10.1248/jhs1956.15.232
出版社：The Pharmaceutical Society of Japan
摘要：Tha micro quantitative determination of Ca in serum with glyoxal bis (2-hydroxyanil) (GBHA) was investigeted. Of many metal ions which are present with Ca in serum, Na, Mg and Pb did not form complex with GBHA. Zinc and Cu are masked by adding 10% NaCN solution to the reaction mixture, Fe and Mn are also masked with 10% triethanolamine. n-Butanol was the most preferable solvent for the extraction of the colored chelate component of Ca with GBHA, because of high sensitivity and no interference with yellow colored serum bilirubin. Methanol and sodium hydroxide-sodium borate buffer were used for the solvent for the preparation of the GBHA reagent solution and for color development, respectively. The stability of the colored chelate in the solution was good. The colorimetric determination of Ca in serum gave good agreements in the recovery of added Ca to serum and in comparison with earlier Clark-Collip's method.