文章基本信息

标题：外用タール色素に関する衛生化学的研究(第1報)逆相分配高速液体クロマトグラフィーによる化粧品用アゾ色素の分析ならびに市販色素中の純色素の定量について
本地全文：下载
作者：早川ゆみ子 ; 宮越護 ; 小林恵美子等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1983
卷号：29
期号：5
页码：274-279
DOI：10.1248/jhs1956.29.5_274
出版社：The Pharmaceutical Society of Japan
摘要：Separation and determination of twenty kinds of cosmetic azo dyes permitted in Japan were studied by reversed-phase high performance liquid chromatography. For identification and determination of the tested dyes, the system of Nucleosil 5C₁₈ column and acidic mobile phase was very useful. The used mobile phases were CH₃CN-THF-H₂O (pH 3.2, H₃PO₄) for oil-soluble dyes, and CH₃CN-THF-H₂O (pH2.0, HClO₄) for water-soluble dyes, Ca-lakes and Ba-lakes, respectively. This method was applied on 93 samples of commercial cosmetic azo dyes for the determination of "pure dye content." Data obtained by this method agreed well with those by the recommended official methods such as titanous chloride titration and spectrophotometric determination. Comparing with the official method, advantage of this method was to prevent the influences of contaminated other dyes, subsidiary dyes and impurities.
关键词：separation and determination of azo dyes;reversed-phase HPLC;oil-soluble dyes;water-soluble dyes;lakes;pure dye content;commercial cosmetic dyes