文章基本信息

标题：食品中の天然色素の分析(第2報)ジアゾメタンによるメチル化反応を利用したコチニール色素の確認
本地全文：下载
作者：山田貞二 ; 野田直希 ; 早川順子等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1985
卷号：31
期号：3
页码：179-185
DOI：10.1248/jhs1956.31.179
出版社：The Pharmaceutical Society of Japan
摘要：Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) were used for the determination of a natural red dye from the scale insect Coccus cacti L. (cochineal) in various foods. Two kinds of orange products appeared by methylation of each of commercial cochineal color preparations and native cochineal color with ethereal diazomethane. These products were employed as reference species to decide whether cochineal color was added in foods or not. Oxalic acid was more effective than hydrochloric acid or acetic acid on the extraction of a color from foods and chromatography on an Amberlite XAD-2 column. A fraction containing a color to be tested was treated with ethereal diazomethane after removal of oxalic acid in the eluate. Orange products in the reaction mixture were detected by silica gel TLC and reversed phase HPLC. This method was found to be very suitable to determine the addition of cochineal color in foods.
关键词：natural dye;cochineal color;diazomethane;oxalic acid;Amberlite XAD-2