文章基本信息

标题：高速液体クロマトグラフィーによる血中パラコートの定量
本地全文：下载
作者：細井要一 ; 神津公
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1985
卷号：31
期号：4
页码：251-255
DOI：10.1248/jhs1956.31.251
出版社：The Pharmaceutical Society of Japan
摘要：Paraquat was directly determined by high performance liquid chromatography (HPLC) with a UV detector using a reversed phase column packed with Nucleosil ₅C₁₈ (4.0 mm×0.15 m) and an ionpairing reagent in the mobile phase. Mobile phase consisted of 0.0075 M sodium 1-hexanesulfonate and 0.06 M sodium perchlorate in 15% methanol-water ; the pH was adjusted to 2.2 with phosphoric acid. Vanillic acid was used as an internal standard. The blood sample was mixed with equal volume of vanillic acid solution and a tenth part of acetic acid to the blood to prevent the choke of the column. After centrifugation, the supernatant was injected to HPLC. Recovery of paraquat obtained from the blood spiked at 50 μg/ml was 106.7±2.5%.
关键词：paraquat;reversed phase HPLC;paired ion;blood