文章基本信息

标题：高速液体クロマトグラフィーによる綿ネルの寝間着中のナフトールAS及び関連色素の定量
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作者：小嶋茂雄 ; 鹿庭正昭 ; 中村晃忠等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1986
卷号：32
期号：5
页码：350-358
DOI：10.1248/jhs1956.32.350
出版社：The Pharmaceutical Society of Japan
摘要：By the collaborative study with Nagoya University Branch Hospital, we have found that naphthol AS (2-hydroxy-3-naphthanilide), an azoic coupling component, might be a causative agent of pigmented contact dermatitis from cotton flannel nightdresses (This journal, 32, 359 (1986)). In the course of the study, we developed a simple method for the determination of naphthol AS in cotton flannels. Naphthol AS could be extracted quantitatively from flannels under reflux with methanol, and purified by silica gel column chromatography by using ethyl acetate as an eluate. After methylation with diazomethane, it could be determined by high performance liquid chromatography (HPLC) by use of the following conditions : column, Nucleosil 50-5 (Nagel, 250 mm×4.6 mm i.d.) ; mobile phase, hexane-chloroform (12 : 5) ; flow rate, 1.0 ml/min ; detector, ultraviolet detector ; detection wavelength, 254 nm. Under these conditions, naphthol AS showed its peak at about 10 min, and its detection limit was found to be about 1 μg/g. Azo dyes in the methanol extracts were also determined by HPLC. Naphthol AS was detected in all cotton flannel nightdresses worn by the patients of pigmented contact dermatitis (contents : 850-11700μg/g, average : 5240μg/g). It was also detected in 5 kinds of commercial products, all of which were cotton flannel nightdresses dyed in the same manner as those worn by the patients (contents : 920-10200μg/g, average : 5760μg/g). It could not be found in other types of flannels. These results could support the idea that naphthol AS may be a causative agent of contact dermatitis from cotton flannel nightdresses.
关键词：cotton flannel nightdress;pigmented contact dermatitis;causative agent;naphthol AS;2-hydroxy-3-naphthanilide;azo dye;high performance liquid chromatograpic determination