文章基本信息

标题：ブロムワレリル尿素の代謝について(第1報)家兎尿からの2-(2-Amino-2-carboxy-ethylthio)-3-methyl-butyrylureaの検出
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作者：礒野秀夫 ; 狐塚寛
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1975
卷号：21
期号：4
页码：188-193
DOI：10.1248/jhs1956.21.188
出版社：The Pharmaceutical Society of Japan
摘要：It is well known that 3-methylbutyrylurea is one of the metabolites of α-bromoisovalerylurea (bromvalerylurea), and the present experiment was carried out in order to find other metabolites of this hypnotic. After the administration of bromovalelrylurea to a rabbit, the urine was collected daily and passed through an anion-exchange resin column. As a result of thin-layer chromatography of the fractions eluted with dil acetic acid, a new Ninhydrin-positive spot was detected much more clearly in the first 24-hr urine than in any other cxcreted urine. From the first 24-hr urine, this substance was obtained as a pure crystalline compound. Mass spectra, infrared spectra, and other chemical analyses suggested that this Ninhydrin-positive spot is a cysteine derivative of bromvalerylurea. Finally, this compound was determined as 2-(2-amino-2-carboxyetylthio)-3-methylbutyrylurea by comparison with a synthetic compound.