文章基本信息

标题：高速液体クロマトグラフィーによる化粧品中のグアイアズレンの定量法
本地全文：下载
作者：鎌田国広 ; 観照雄 ; 原田裕文等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1984
卷号：30
期号：3
页码：144-148
DOI：10.1248/jhs1956.30.3_144
出版社：The Pharmaceutical Society of Japan
摘要：A procedure is described for the determination of guaiazulene (GA) in cosmetics by high performance liquid chromatography (HPLC). The sample was mixed with Cellite, transfered to a chromatographic column, and eluted with acetonitrile. The acetonitrile was added to water, and liquid-liquid partition between hexane. The hexane was concentrated and then GA was determined by HPLC on octadecylsilated silica column using a mobile phase of methanol-water (9 : 1, v/v), UV detector (284 nm) and fluorescence spectrophotometric detector (FD) (Ex·λ : 285 nm, Em·λ : 390 nm) for detection. As a result, the calibration curve was linear in the range 1-10 μg/ml of GA on FD and UV detector. Recoveries of GA obtained from a variety of cosmetic samples spiked at 50 and 100 ppm levels were 93.8-97.2% and 94.8-97.0%, respectively. When this method was applied to the analysis of GA in commercial samples, GA could be determined without the influence of obstructive substances.
关键词：guaiazulene;HPLC;celite column;cosmetic;guaiazulene determination