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标题：Some Properties of Metallothioneins from Hepato-pancreas and Kidney in Carp (Cyprinus carpio)
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作者：HIDEAKI KITO ; YOUKI OSE ; KYOZO HAYASHI 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1984
卷号：30
期号：3
页码：119-125
DOI：10.1248/jhs1956.30.3_119
出版社：The Pharmaceutical Society of Japan
摘要：Carp (Cyprinus carpio) were injected intraperitoneally with 2 mg Cd/kg body weight as CdCl₂ solution. Two types of isometallothioneins were separated from carp kidney by successive treatment with homogenization, ultracentrifugation, incubation, centrifugation, gel filtration and ion exchange chromatography. Purity was shown by polyacrylamide DISC-gel electrophoresis. The molecular weight of native metallothionein-I and -II was found to be 12000-13000. The disappearance of Cd-mercaptide bond was estimated under acidic conditions. Cysteic residues were about 30% in kidney isometallothioneins, Gly and Lys were high, but no aromatic amino acids or His residues were detected. In a double diffusion test, spur formation was shown between two hepato-pancreatic isometallothioneins, and also between hepato-pancreatic metallothionein-II and kidney metallothionein-II.
关键词：metallothionein;carp (Cyprinus carpio);kidney;amino acid composition;spurformation;antigenic determinant