文章基本信息

标题：コイ(Cyprinus carpio)における肝膵臓と腎臓中メタロチオネインの免疫組織学的局在
本地全文：下载
作者：鬼頭英明 ; 小瀬洋喜 ; 三谷一憲等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1984
卷号：30
期号：4
页码：194-199
DOI：10.1248/jhs1956.30.4_194
出版社：The Pharmaceutical Society of Japan
摘要：Antiserum to carp (Cyprinus carpio) hepato-pancreatic MT-II was prepared by use of New Zealand white rabbit. Immunoglobulin G (IgG) was separated on a Protein A Sepharose CL-4B column, and labelled with horseradish peroxidase (HRP). The slices of hepato-pancreas and kidney from carps of the two groups, non-injected and Cd-injected, were prepared. Metallothionein in the slices reacted with the IgG by direct method. In the hepato-pancreas of carp injected with CdCl₂, the staining for metallothionein was observed in nucleus of hepatocytes, sinusoids, intracellular space and acinar cells in pancreas tissue, the nucleus and acinar cells being stained remarkably. In a non-injected carp, the acinar cells in pancreas tissue were stained slightly, suggesting the presence of metallothionein. This result indicated that (Cu, Zn)-metallothionein in hepato-pancreas from a non-injected carp was present mainly in the acinar cells in pancreas tissue. In the kidney slices prepared from non-injected and Cd-injected carps, the staining for metallothionein was observed in the eprithelium of neck segment, proximal convoluted segment II and distal convoluted segment, but was not found in the glomeruli. It was proved that (Cu, Zn)-metallothionein also exists in the kidney of a non-injected carp.
关键词：metallothionein;immunoglobulin G;immunohistological localization;direct method;horseradish peroxidase;hepatocyte nucleus;pancreas tissue acinar cells;proximal convoluted segment II;carp;Cyprinus carpio