文章基本信息

标题：高速液体クロマトグラフィーによる化粧品中のエストラジオール, エチニルエストラジオールの分離定量法
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作者：鎌田国広 ; 山添律子 ; 原田裕文等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1980
卷号：26
期号：1
页码：41-45
DOI：10.1248/jhs1956.26.41
出版社：The Pharmaceutical Society of Japan
摘要：Several experimental conditions for the determination of estradiol (ED) and ethynylestradiol (EED) in cosmetics by high-speed liquid chromatography (HSLC) were developed. ED and EED were extracted from samples with dichloromethane, the extract was concentrated, placed on a silica gel column, and the column was washed with 1, 2-dichloroetane. The fraction eluted with ethyl acetate was concentrated and then ED and EED were determined by HSLC. The conditions for HSLC were as follows : Column, Zorbax SIL (250×2.1 mm, i.d.) ; mobile phase, tetrahydrofuran-hexane (1 : 9) ; flow rate, 0.75 ml/min ; column temp, room temp ; detector, fluorescence spectrophotometer (exciting wave length, 286 nm, fluorescence wave length, 307 nm). The detection limit of ED and EED was 0.005 μg/ml. The recovery of ED added to cosmetic samples was 92.2-96.0% and that of EED was 92.6-96.4%.
关键词：cosmetic;estradiol;ethynyl estradiol;high speed liquid chromatography;silicagel column chromatography