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标题：Action of n-Butyl p-Hydroxybenzoate on the Cytoplasmic Membrane of Yeast
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作者：TATSURO MIYAHARA ; TATSUO SAKAI
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1980
卷号：26
期号：3
页码：118-122
DOI：10.1248/jhs1956.26.118
出版社：The Pharmaceutical Society of Japan
摘要：When washed cells of Kloeckera apiculata were suspended in n-butyl p-hydroxybenzoate solution, release of α-amino nitrogen from the cells into the medium was observed. There was also a release of thiamine into the medium on incubation of thiamine-accumulated cells with n-butyl p-hydroxybenzoate. A close correlation between the extent of leakage and the percentage of dead cells was found. The cell uptake of thiamine was inhibited at a concentration of n-butyl p-hydroxybenzoate that caused no release of α-amino nitrogen. Formation of protoplasts on incubation of the benzoate-treated cells with lytic enzyme was suppressed in comparison with intact cells. Protoplasts were lysed by n-butyl p-hydroxybenzoate on incubation of the protoplasts with the benzoate in 0.8M NaCl solution. In view of these results, it seems possible that n-butyl p-hydroxybenzoate attacks the cytoplasmic membrane of yeast.
关键词：n-butyl p-hydroxybenzoate;Kloeckera apiculata;α-amino nitrogen release;thiamine uptake;protoplast;lytic enzyme