文章基本信息

标题：ゲル濾過を用いた食品添加物カラギーナン中の単糖, 二糖の定量
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作者：荒川正一 ; 早川清子 ; 伊藤正夫等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1985
卷号：31
期号：2
页码：137-140
DOI：10.1248/jhs1956.31.137
出版社：The Pharmaceutical Society of Japan
摘要：The identification and determination of cabohydrates in low molecular fraction and determination of molecular weight of carrageenans in high molecular fraction of 5 carrageenans of food additives separated by Sephacryl S-300 column chromatography were studied. Lactose, glucose or sucrose was identified by thin-layer chromatography (TLC) as low molecular weight carbohydrates in carrageenan of food additives. By the phenol-sulfuric acid method by use of saccharide identified by TLC as a standard, the contents of mono-and disaccharides in carrageenans of food additives obtained from low molecular fraction separated by Sephacryl gel filtration, outer solution dialysed with cellophane tube and extraction with 80% methanol were found to be within the range of 20-60%. The molecular weight was more than 100000 for each of carrageenans in carrageenans of food additives by Sephacryl S-300 chromatography with well-characterized dextrans. Sulfate contents of carrageenans by the barium sulfate gravimetric method were found to be above 18%, except one to be 7.5%.
关键词：carrageenan;gel filtration;sulfate;TLC;phenol-sulfuric acid method;molecular weight