文章基本信息

标题：血清中パラコートの迅速簡易定量法
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作者：三浦修 ; 佐々木智 ; 佐々木司郎等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1986
卷号：32
期号：3
页码：167-170
DOI：10.1248/jhs1956.32.167
出版社：The Pharmaceutical Society of Japan
摘要：A simple and rapid method for the determination of paraquat in the serum was developed by a modification of the method originally reported by Knepil. Serum samples were treated with a mixture of chloroform-ethanol and ammonium sulfate and centrifuged to obtain a clear aqueous paraquat solution. Alkaline dithionite solution was added to the supernatant and the absorbance at 396 nm was recorded as A. A small amount of acetic acid was added to the same cuvette to distinguish the coloured paraquat radical and the absorbance at 396 nm resulted from the serum constituents was again measured as B. From the difference between A and B, the concentration of paraquat was determined in the range of 0.1-4 μg/ml serum, with the recovery of about 90%. This method posesses the advantages of 1) no need of special instrument and 2) short time for the determination. By using this method, paraquat concentration in the serum was monitored succesively during 7 d in a poisoning case.
关键词：paraquat;determinaton;colorimetry;acute paraquat poisoing;drug monitoring;serum