文章基本信息

标题：ヒ素化合物の細胞毒性とDNA修復に及ぼす影響
本地全文：下载
作者：奥井登代
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1987
卷号：33
期号：1
页码：20-24
DOI：10.1248/jhs1956.33.20
出版社：The Pharmaceutical Society of Japan
摘要：Cytotoxicity and effects on ultraviolet (UV) killing to Chinese hamster ovary (CHO) cells of arsenic compounds were examined by means of clonogenic survival assay, and the effects on DNA repair in CHO cells of arsenic compounds were examined by unscheduled DNA synthesis (UDS). Inorganic arsenic compounds were more lethal to CHO cells than organic arsenic compounds. Furthermore, arsenite was much more cytotoxic to CHO cells than arsenate. Treatment with inorganic arsenic compounds after UV irradiation enhanced UV killing to CHO cells. These effects were observed even when the cells were treated with arsenic for 1 h after UV irradiation. However, when the cells were treated with arsenic at 18 h after UV irradiation, arsenic had little effect on the UV killing to CHO cells. On the other hand, treatment with organic arsenic compounds caused no obvious potentiation of UV killing to CHO cells. The UV-induced UDS decreased by treatment with arsenite for 4 h after UV irradiation, it decreased a little with arsenate. However, when the cells were treated with arsenate for 2 h before UV irradiation, arsenate caused reduction of the UV-induced UDS. These results suggest that inorganic arsenic compounds inhibit DNA repair.
关键词：arsenic compound;CHO cell;cytotoxicity;unscheduled DNA synthesis;DNA repair