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标题：INTERACTION OF SAPONINS WITH RED BLOOD CELLS AS WELL AS WITH THE PHOSPHATIDYLCHOLINE LIPOSOMAL MEMBRANES
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作者：TATSUNOSUKE NAKAMURA ; KEIZO INOUE ; SHOSHICHI NOJIMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1979
卷号：2
期号：6
页码：374-382
DOI：10.1248/bpb1978.2.374
出版社：The Pharmaceutical Society of Japan
摘要：Various purified saponins were examined for their lytic activities against mammalian erythrocytes and their activities to interact with liposomal membranes, and were classified into at least five categories according to their reactivities. 1. Digitonin, F-gitonin, dioscin and paris saponin Pa : These saponins showed hemolytic activity and induced permeability change in liposomal membranes containing cholesterol. The interaction of these saponins with cholesterol was "irreversible". The interaction was independent of acyl chain lengths of phosphatidylcholine. 2. Gracillin : This saponin showed hemolytic activity but did not induce permeability change of liposomal membrane with cholesterol, except those containing distearoyllecithin. The interaction of gracillin with cholesterol was "irreversible". 3. Akebia saponin B, C, P_D and P_G : These showed hemolytic activities and induced the permeability changes of liposomes with cholesterol. Their activities to induce permeability changes were affected by chain length of phosphatidylcholine. With increase of chain length, the sensitivity of liposomes toward these saponins decreased. The interaction of saponins with cholesterol was "reversible". 4. Chikusetsusaponin III : This saponin has a weak lytic activity against erythrocytes and has the activity to induce permeability change of liposomes without cholesterol. Cholesterol in the phosphatidylcholine liposomes depressed the sensitivity of the liposomal membrane to the saponin. 5. Chikusetsusaponin V and akebia saponin E and Pk : These compounds ("bisdesmosidisch") showed neither detectable activity to lyse erythrocytes nor to damage liposomal membranes. liposomal membranes.
关键词：hemolysis