文章基本信息

标题：ポリビニルアルコール系カラムを用いた高速液体クロマトグラフィーによる尿中メタンフェタミンの分析
本地全文：下载
作者：細井要一
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1987
卷号：33
期号：3
页码：170-173
DOI：10.1248/jhs1956.33.170
出版社：The Pharmaceutical Society of Japan
摘要：Methamphetamine in the human urine wasdirectly determined by high performance liquid chromatography (HPLC) by using a polyvinylalcohlic column and alkali mobile phase. The detailed conditions were as follows : column : Asahipak GS-320M 7.6 mm i.d.×100 mm, column temp. : 40°C, mobile phase : 80% {0.02 M NaHCO₃+0.02 M Na₂CO₃ (8 : 2)} +20% CH₃CN, flow rate : 1.0 ml/min, detector : UV photometer (207 nm). The urine was directly injected into HPLC. Methamphetamine was eluted by adsorption chromatography, and separated from endogenous urine constituents. Determination of methamphetamine was carried out successfully by using the calibration curve by peak high (1-10μg/ml). The recovery of methamphetamine added to the human urine (5μg/ml) was 92.0±1.0%.
关键词：methamphetamine;adsorption HPLC;human urine;polyvinylalcohlic column;alkali mobile phase