文章基本信息

标题：IN VITRO O-METHYLATION OF 4-HYDROXYESTRONE MONOSULFATES
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作者：KAZUTAKE SHIMADA ; YOSHIKO YUMURA ; TOSHIO NAMBARA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1982
卷号：5
期号：6
页码：448-450
DOI：10.1248/bpb1978.5.448
出版社：The Pharmaceutical Society of Japan
摘要：In vitro O-methylation of 4-hydroxyestrone monosulfates has been examined by means of high-performance liquid chromatography with electrochemical detection. When 4-hydroxyestrone or its 3-sulfate was incubated with rat liver homogenate in the presence of S-adenosyl-L-methionine, the 4-methyl ether was formed in twelve times larger amount than the 3-methyl ether. 4-Hydroxyestrone 4-sulfate served for O-methylation to much less extent as a substrate namely, only a small amount of the 4-methyl ether was formed. Enzymic sulfation of 4-hydroxyestrone with rat liver 105000 g supernatant fortified with adenosine 3'-phosphate 5'-phosphosulfate provided solely catechol estrogen 4-sulfates. The participation of catechol O-methyltransferase and aryl sulfatase in the formation of guaiacol estrogens has been discussed.
关键词：electrochemical detection