文章基本信息

标题：STUDIES ON ANTITUMOR POLYSACCHARIDES OF FLAMMULINA VELUTIPES (CURT. EX FR.) SING.II. THE STRUCTURE OF EA3 AND FURTHER PURIFICATION OF EA5
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作者：TETSURO IKEKAWA ; YOSHIAKI IKEDA ; YUKO YOSHIOKA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1982
卷号：5
期号：8
页码：576-581
DOI：10.1248/bpb1978.5.576
出版社：The Pharmaceutical Society of Japan
摘要：An antitumor polysaccharide, EA₃ isolated from a Japanese edible mushroom, Flammulina velutipes (CURT. ex FR.) SING.^1-3) is composed of D-glucose. Studies to determine its structure were performed by mean of partial acid hydrolysis, acetolysis and the chemical analysis of the complete hydrolysates of the fully methylated polysaccharide. Thus the chemical structure of EA₃ was found to be that of a β-(1→3)-glucan. Another antitumor polysaccharide (EA₅) also isolated from F.velutipes^{2, 3)} was fractionated on a concanavalin A-Sepharose 4B column. The active antitumor component was further purified by column chromatography on Sephadex G-200. Among the polysaccharides isolated the highest molecular weight polysaccharide (EA₅₀₁) showed the highest rate of antitumor activity. The component sugar of EA₅₀₁ were found to be D-glucose 42.3%, D-galactose 17.3%, D-mannose 12.2%, D-xylose 6.7% and L-arabinose 14.7%.
关键词：F.velutipes