文章基本信息

标题：EFFECTS OF VARIOUS PROTEASE INHIBITORS ON PHAGOCYTOSIS OF ANTIGEN-ANTIBODY COMPLEX BY MACROPHAGES
本地全文：下载
作者：ICHIRO FUKAZAWA ; JIRO KOYAMA
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1982
卷号：5
期号：9
页码：699-707
DOI：10.1248/bpb1978.5.699
出版社：The Pharmaceutical Society of Japan
摘要：In order to characterize proteases of macrophages involved in phagocytosis, the effects of various protease inhibitors were investigated in measuring the uptake and digestion of Bacillus subtilis α-amylase complex of guinea pig IgG2 antibody by homologous peritoneal macrophages. The inhibitory effects were observed with diisopropyl fluorophosphate (DFP), L-1-p-tosylamido-2-phenylethyl chloromethyl kerone (TPCK), 1-chloro-3-tosylamido-7-amino-L-2-heptanone (TLCK), antipain, chymostatin, elastatinal and leupeptins, but not with pepstatins. DFP and TPCK reduced both the amounts of antigen-antibody (Ag-Ab) complex taken up by macrophages and of digested products released from the cells. TLCK, antipain, chymostatin, elastatinal and leupeptins also reduced the amount of digested products, but increased that of Ag-Ab complex associated with macrophages. These results suggest that the inhibitory mechanism of DFP and TPCK toward the digestion of soluble Ag-Ab complex by macrophages differs from those of other inhibitors examined.
关键词：leupeptins