文章基本信息

标题：Enhancement of Heparin-Binding Ability of Fibronectin by S-Carboxamidemethylation
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作者：Yasuhiro KOHAMA ; Takefumi MIYAMOTO ; Hiroaki OKA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1989
卷号：12
期号：10
页码：626-633
DOI：10.1248/bpb1978.12.626
出版社：The Pharmaceutical Society of Japan
摘要：Human plasma fibronectin (FN) was reduced and carboxamidemethylated, and its binding ability to several matrices was analyzed in vitro. The binding of S-carboxamidemethyl (Cam)-FN to heparin-Sepharose was not influenced by either 4M urea, 0.5 M NaCl or 0.5% heparin, but was disrupted by the coexistence of urea and NaCl or heparin. S-Cam-FN, compared with intact FN, obviously had a more potent ability to bind heparin, while it had little or no binding ability to gelatin, fibrin and thrombin-stimulated platelets. A conformational change of S-Cam-FN by heparin-binding has been proposed as a possible mechanism from the result of circular dichroic spectrum measurement.
关键词：circular dichroic spectrum