文章基本信息

标题：Effects of Mushroom Toxins on Glycogenolysis : Comparison of Toxicity of Phalloidin, α-Amanitin and DL-Propargylglycine in Isolated Rat Hepatocytes
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作者：Akiko KAWAJI ; Kiyotaka YAMAUCHI ; Shinobu FUJII 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1992
卷号：15
期号：3
页码：107-112
DOI：10.1248/bpb1978.15.107
出版社：The Pharmaceutical Society of Japan
摘要：The effects of phalloidin and α-amanitin as toxins of Amanita species and DL-propargylglycine identified from A. abrupta on the glycogenolysis in isolated rat hepatocytes were investigated. Phalloidin decreased glycogen content and activated phosphorylase α activity remarkably. α-Amanitin also decreased glycogen content significantly but activated phosphorylase α activity slightly. DL-Propargylglycine slightly affected glycogenolysis. Phalloidin, which most affected glycogenolysis among the three compounds mentioned above, elevated cytosolic Ca²⁺ concentration ([Ca²⁺]_i) and ⁴⁵Ca uptake into cells. Phalloidin depressed slightly ³H-inositol incorporation into phosphatidylinositol (PI) and remarkably phosphatidylinosito 4, 5-bisphosphate (PIP₂) but increased phosphoinositides breakdown. These results suggest that phalloidin alters phosphoinositides turnover and intracellular Ca²⁺ homeostasis, subsequently activates phosphorylase α, resulting in glycogenolysis in isolated rat hepatocytes.
关键词：DL-propargylglycine