文章基本信息

标题：Effect of Benzylidene Derivative (Novel Antirheumatic Agent) on Chondrocyte Metabolism
本地全文：下载
作者：Koju WATANABE ; Fumihiko KIMURA ; Masayuki SHINMEI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1992
卷号：15
期号：5
页码：239-246
DOI：10.1248/bpb1978.15.239
出版社：The Pharmaceutical Society of Japan
摘要：The effects of protocatechualdehyde (PAL), one of the metabolites of 3, 4-diacetoxy benzylidene diacetate (ACP), on proteogylcan metabolism and secretion of interleukin 1 (IL-1) like activity (lymphocyte activating factor ; LAF activity) were studied using rabbit articular chondrocytes culture under phorbol myristate acetate (PMA) and Ca²⁺ ionophore A23187 (A23187) or IL-1α stimulation. In IL-1α (20 u/ml) or PMA (0.1 μg/ml) and A23187 (0.2 μg/ml) treated culture of rabbit articular chondrocytes, PAL significantly reduced the degradation of ³⁵S-proteoglycan (³⁵S-PG) from the cells and matrix layers into the culture media in a dose dependent fashion without affecting proteoglycan synthesis. Similarly, the secretion or production of matrix metalloproteinases which degrade proteoglycans was also inhibited to the same extent under IL-1α stimulated condition. However, PAL caused no effect on the secretion of IL-1 like activity by chondrocytes. These results suggest that an attractive candidate for an anti-inflammatory drug, ACP, which is a prodrug of PAL, has also a favorable action on chondrocyte metabolism in terms of proteoglycan degradation via inhibition of matrix metalloproteinases secretion or production.
关键词：interleukin 1