文章基本信息

标题：EFFECT OF LIPID PEROXIDATION ON COVALENT BINDING OF ACTIVE METABOLITE OF ACETAMINOPHEN TO LIVER MICROSOMAL MACROMOLECULES IN SEVERAL ANIMAL SPECIES
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作者：KAZUO AIKAWA ; TETSUO SATOH ; KEIKO KOBAYASHI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1978
卷号：1
期号：1
页码：22-27
DOI：10.1248/bpb1978.1.22
出版社：The Pharmaceutical Society of Japan
摘要：There was good correlation of lipid peroxidation and covalent binding of acetaminophen (AAP) to liver microsomal protein in mice, guinea pigs and rabbits, but not in rats. By increasing concentrations of EDTA which is an inhibitor of lipid peroxidation, the degree of microsomal lipid peroxidation decreased in mice, guinea pigs, rabbits and rats, and the amount of covalent binding of AAP metabolite to liver microsomal protein increased in mice, guinea pigs and rabbits, but no consistent results were observed in rats. GSH depletion caused by AAP metabolite was increased in accordance with increase in the concentration of EDTA in mice and rats. In rats, the metabolism of AAP increased with increasing concentrations of EDTA and, in contrast to liver microsomes, the radioactivity of enzyme-mediated ¹⁴C-AAP metabolite in liver microsomal lipid was not observed. These results indicated that the decrease in covalent binding for AAP metabolite to liver microsomal protein with increasing concentrations of EDTA was not due to the decrease in the amount of AAP metabolite and the binding of ¹⁴C-AAP metabolite to liver microsomal lipid layer.
关键词：GSH depletion