文章基本信息

标题：ASSIGNMENT OF CIRCULAR DICHROISM SPECTRA OF 2-(4'-HYDROXYPHENYLAZO) BENZOIC ACID INDUCED BY BOVINE SERUM ALBUMIN TO THE AZO FORM
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作者：TAMIKO SAKURAI ; SEISHI TSUCHIYA ; HIDEO MATSUMARU 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1981
卷号：4
期号：6
页码：451-454
DOI：10.1248/bpb1978.4.451
出版社：The Pharmaceutical Society of Japan
摘要：In the displacement study, the effects of tolbutamide on the absorption spectra and induced CD spectra of 2-(4'-hydroxyphenylazo) benzoic acid (HABA)-bovine serum albumin (BSA) complex were investigated using the samples obtained from equilibrium dialysis at pH 7.40. The binding of tolbutamide to BSA enhanced the binding of the azo form in spite of the displacement of the hydrazone form of bound HABA at the molar ratio of HABA to BSA of 0.65, causing the increase in the negative ellipticity at 445 nm, which was termed the C band. The C band could be assinged to the azo form of bound HABA.
关键词：hydrazone form