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标题：Specific Binding of Interferon-γ and-α2a to Tumor Cells and Their Antitumor Activities
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作者：Yoshiaki ISHII ; Shigeru TSUKAGOSHI
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1988
卷号：11
期号：7
页码：465-471
DOI：10.1248/bpb1978.11.465
出版社：The Pharmaceutical Society of Japan
摘要：Binding activities of a recombinant human interferon-γ (IFN-γ) preparation with 5 human tumor cell lines, including lung large cell carcinoma PC-13, lung small cell carcinoma QG-90, amelanotic melanoma HMV-1, renal carcinoma ACHN and Burkitt lymphoma Daudi, were compared with those of IFN-α_2a. Three out of five human tumor cell lines, ACHN, HMV-1, and QG-90, were highly susceptible to IFN-γ : the concentrations required to inhibit 50% of the growth of cells (IC₅₀) of ACHN, HMV-1, and QG-90 were 26, 11 and 7 pM, respectively. IFN-α_2a also markedly inhibited the growth of ACHN, HMV-1 and Daudi cells : the values of IC₅₀ for the former and latter cells were 20, 31 and 0.8 pM, respectively. With these susceptible cells, larger amounts of IFN_-γ were bound than with resistant cells. On the other hand, IFN-α_2a bound all cell lines, irrespective of the susceptibilities of cells. The values of the apparent dissociation constant (K_d) of binding of both IFNs ranged from 1.3 to 7.3×10^-10M. The competitive binding test revealed that the binding properties of IFN_-γ were different from those of IFN-α_2a. Under the present experimental condition, it could be said that, with IFN_-γ, the susceptibilities of cells were linked of the expression of the receptors, but there were some exceptions with IFN-α_2a.
关键词：human tumor