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标题：Different Effects of Cinnamic Acid on the O2-Generation by Guinea Pig Macrophages Stimulated with a Chemotactic Peptide and Immune Complex
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作者：Tomozumi IMAMICHI ; Tohoru NAKAMURA ; Kouji HAYASHI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1990
卷号：13
期号：6
页码：344-352
DOI：10.1248/bpb1978.13.344
出版社：The Pharmaceutical Society of Japan
摘要：Cinnamic acid inhibits the O^-₂-generating response of guinea pig peritoneal macrophages elicited with a chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (fMLP), but not those with ovalbumin complex of immunoglobulin G₂ antibody and phorbol-myristate acetate. ¹⁾During the course of study on the inhibitory mechanism of cinnamic acid, we found that the acid also inhibited the Ca²⁺ mobilization elicited with fMLP, but not that with the immune complex. In addition, the treatment of macrophages with Ionomycin and ethyleneglycol bis-(β-aminoethylether)-N, N'-tetraacetic acid for depletion of the intracellular Ca²⁺ inactivated completely the O^-₂generation elicited with fMLP, but not its counterpart of the immune complex. Thus, the inhibitory activity of cinnamic acid on the O^-₂generation elicited with fMLP seems partly due to that on the Ca²⁺ mobilization. On the other hand, cinnamic acid augmented the intracellular accumulation of adenosine 3', 5'-cyclic monophosphate (cyclic AMP) in the presence of 3-isobutyl 1-methylxanthine (IBMX), and elevated more intensively the concentration of cyclic AMP when macrophages were stimulated with fMLP. Since IBMC inhibited the O^-₂generation elicited with fMLP, the enhancement of activation of an adenylate cuclase by cinnamic acid might cause depression of the O^-₂generation. This possibility, however, seems to be excluded by the fact that the same effect of cinnamic acid was observed even when macrophages were stimulated with the immune complex.
关键词：cyclic AMP