文章基本信息

标题：Fluorogenic Bimane Substrates with Dabsyl Group for Endopeptidases : Chymotrypsin, Collagenase and Thermolysin
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作者：Eisuke SATO ; Hiromi HATTORI ; Yuichi KANAOKA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1991
卷号：14
期号：10
页码：599-604
DOI：10.1248/bpb1978.14.599
出版社：The Pharmaceutical Society of Japan
摘要：It was found that the fluorescence of 9, 10-dioxa-syn-3, 4, 6, 7-tetramethylbimane (bimane) can be quenched in the presence of dimethylaminoazobenzensulfonyl (Dabsyl) group. New combination of bimane (fluorophor) and dabsyl group (quencher) was applied to the syntheses of intramolecularly quenched fluorogenic substrates for hydrolytic enzymes. Bimane peptides containing dabsyl group were prepared, and were shown to be useful fluorogenic substrates for the assay of endopeptidases such as chymotrypsin, collagenase and thermolysin
关键词：thermolysin