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标题：DEGRADATION OF HISTAMINE IN THE PRESENCE OF ASCORBIC ACID AND Cu2+ ION : INVOLVEMENT OF HYDROGEN PEROXIDE
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作者：ITARU YAMAMOTO ; HITOSHI OHMORI
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1981
卷号：4
期号：1
页码：15-19
DOI：10.1248/bpb1978.4.15
出版社：The Pharmaceutical Society of Japan
摘要：In the presence, but not in the absence of Cu²⁺, ascorbate decomposes histamine in citrate phosphate buffer (pH 6.5) at 37°, but not at 0°. The breakdown is completely inhibited by catalase, but only slightly by superoxide dismutase, and scavengers of OH·like benzoic acid, ethanol or potassium iodide. A¹O₂ scavenger, α-tocopherol also did not show significant effects on the reaction. On the other hand, addition of H₂O₂ to the reaction mixture markedly enhances the rate of histamine breakdown induced by ascorbate or ascorbate-Cu²⁺ systems. However, H₂O₂ alone cannot breakdown histamine even in the presence of Cu²⁺. Histamine breakdown induced by ascorbate appears to be dependent upon the autooxidation of this vitamine. From these results and the findings reported by Chatterjee et al. that the products of its aerobic oxidation, dehydroascorbic acid and H₂O₂ were ineffective in reacting with histamine in the presence of Cu²⁺, it is concluded that the combination of H₂O₂ and the intermediate of ascorbate oxidation (monodehydroascorbic acid or other unstable species), both of which are produced during the autooxidation of ascorbate, plays a major role in the histamine transformation by ascorbate-Cu²⁺ system.
关键词：H2O2