文章基本信息

标题：EFFECTS OF p-CHLOROMERCURIBENZENE SULFONATE ON THE UPTAKE OF D- AND L-LEUCINES IN EHRLICH ASCITES TUMOR CELLS
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作者：RENSUKE GOTO ; SATOSHI HANAMURA ; OSAMU TAMEMASA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1982
卷号：5
期号：10
页码：780-788
DOI：10.1248/bpb1978.5.780
出版社：The Pharmaceutical Society of Japan
摘要：In order to ascertain the significane of the hydrophobic region in the carrier protein responsible for the D-leucine transport reported previously, the effects of p-chloromercuribenzene sulfonate (PCMBS) were investigated on the transport of D- and L-leucines in Ehrlich ascites tumor cells. The uptake of D-leucine in the presence of PCMBS was inhibited more strongly than that of L-leucine. K_m values for D-leucine uptake increased with increasing concentration of PCMBS, while those for L-leucine showed only a little increase. D-Leucine uptake was restored considerably from the PCMBS inhibition by washing with buffer, whereas L-leucine uptake was only slightly affected. The inhibition of D-leucine exit by PCMBS was also higher than that of L-leucine. These results suggest that the binding site of PCMBS for the carrier protein of Ehrlich cells is involved in its hydrophobic region that would be more significant for the binding of alkyl side chain of D-leucine than that of L-leucine.
关键词：uptake of amino acid