文章基本信息

标题：DETERMINATION OF PROSCILLARIDIN IN PLASMA BY RADIOIMMUNOASSAY
本地全文：下载
作者：KENJI KOJIMA ; KAZUMITSU YAMAMOTO ; YOSHIAKI TERAUCHI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1986
卷号：9
期号：8
页码：665-671
DOI：10.1248/bpb1978.9.665
出版社：The Pharmaceutical Society of Japan
摘要：The development of a sensitive and selective radioimmunoassay for determination of proscillaridin in plasma is described. Antiserum against proscillaridin was obtained from guinea pig immunized with an immunogen prepared by conjugating methylproscillaridin, 14β-hydroxy-3β-[(4-O-methyl-α-L-rhamnosyl) oxy] bufa-4, 20, 22-trienolide to bovine serum albumin. Methyl N-[3-[(14β-hydroxybufa-4, 20, 22-trienolid-3β-yl) oxy-carbonyl] propanoyl]-L-[3', 5'-¹²⁵I₂] diiodotyrosinate ([¹²⁵I] SST) was used as a radioactive ligand. [¹²⁵I] SST and antiserum were added to the recovered sample from plasma using a Bond Elut^[○!R] column. After overnight incubation, the antibody-bound and free [¹²⁵I] SST were separated using polyethylene glycol. The mean coefficients of variation of intra and interassay at four different plasma concentrations were 4.0 and 5.0%, respectively. The assay was able to determine as little as 125 pg/ml of proscillaridin in plasma by using 1.5 ml of sample. Beagle dogs were orally administered one tablet (Talusin^[○!R]) containing 0.25 mg of proscillaridin. Proscillaridin was rapidly absorbed, exhibiting plasma maximum concentration of 2.06 ng/ml at 20 min. Thereafter, the plasma level declined biphasically with half-lives of 0.6 and 25.4 h.
关键词：Talusin[○!R]