文章基本信息

标题：CHARACTERIZATION OF HETEROLOGOUS DESENSITIZATION OF RAT RETICULOCYTE ADENYLATE CYCLASE SYSTEM
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作者：ATSUSHI YAMASHITA ; TOMONORI KUROKAWA ; TOSHIO DANURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1987
卷号：10
期号：6
页码：250-254
DOI：10.1248/bpb1978.10.250
出版社：The Pharmaceutical Society of Japan
摘要：Treatment of rat reticulocytes with isoproterenol caused about 50, 25, and 25% decreases in β-adrenergic agonist-, fluoride-, and guanine nucleotide-stimulated adenylate cyclase activities, respectively. The desensitization was also induced by dibutyryl adenosine 3', 5'-cyclic monophosphate (cyclic AMP) and 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) prevented the isoproterenol-induced desensitization, suggesting the involvement of cyclic AMP in the desensitization. Time course studies revealed that the desensitization to NaF-AlCl₃ occurred faster than that to isoproterenol. Furthermore, the rate of the resensitization to NaF-AlCl₃ by removal of isoproterenol was also faster than that to isoproterenol. Thus, it is likely that both guanine nucleotide-binding stimulatory regulatory protein, N_s, and β-adrenergic receptor are sequentially involved in both desensitization and resensitization of the adenylate cyclase system in rat reticulocytes to isoproterenol.
关键词：rat reticulocytes