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  • 标题:ENZYME IMMUNOASSAY FOR METHAMPHETAMINE
  • 本地全文:下载
  • 作者:KIMIKO AOKI ; YUKIO KUROIWA
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:1983
  • 卷号:6
  • 期号:1
  • 页码:33-38
  • DOI:10.1248/bpb1978.6.33
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:A competitive enzyme immunoassay for methamphetamine with alkaline phosphatase labeled methamphetamine, Sepharose-antibody and p-nitrophenylphosphate as substrate was developed. The anti-methamphetamine antisera produced in rabbits by immunization with N-(4-aminobutyl) methamphetamine-BSA conjugate were specific for methamphetamine and showed low cross-reactivities with p-OH methamphetamine and amphetamine (metabolites of methamphetamine). The range of methamphetamine measurable by the enzyme immunoassay was 1 to 300 ng/tube. According to the assay, methamphetamine could be detected from urine and extract of hair.
  • 关键词:hair
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