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标题：RADIOIMMUNOASSAY OF THYROTROPIN-RELEASING HORMONE (TRH) IN RAT, DOG, AND HUMAN BLOOD
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作者：SHIGEHARU YOKOHAMA ; KENJI YAMASHITA ; HAJIME TOGUCHI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1983
卷号：6
期号：9
页码：627-636
DOI：10.1248/bpb1978.6.627
出版社：The Pharmaceutical Society of Japan
摘要：A radioimmunoassay was developed for the measurement of exogenous thyrotropin-releasing hormone (TRH) in the whole blood of rats, beagle-dogs and humans. Even at low temperature, TRH is degraded so quickly in the whole blood that stabilization of TRH in the blood is necessary. For this purpose, the direct extraction with methanol and concentration before the radioimmunoassay was satisfactorily performed. The method is highly sensitive so that a lower quantifiable concentration of 20 pg/ml was detectable, and good reproducibility and standard errors of less than 10% from triplicate standard curves were obtained. At low concentration of TRH, the effect of food ingestion and volume of whole blood on the sensitivity of the radioimmunoassay was observed and thin layer chromatographic treatment improved it.
关键词：human whole blood