文章基本信息

标题：LIMB DEFORMITY INDUCED IN CHICK EMBRYO BY HYDROXYUREA
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作者：MASAHIKO IWAMA ; YOSHIMITSU SAKAMOTO ; ATSUSHI HONDA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1983
卷号：6
期号：11
页码：836-843
DOI：10.1248/bpb1978.6.836
出版社：The Pharmaceutical Society of Japan
摘要：After a dose of 800 μg of hydroxyurea (HU) was administered to day-4 chick embryos in ovo, the limb deformity including micromelia was observed. We examined the teratogenic mechanism of the action for HU by using histochemical and biochemical techniques. The present study provides histological evidence for the cell death and the depression of cell proliferation just after the treatment with HU and the retardation in periosteal ossification during the of repair and/or recovery from cell death. On the other hand, the incorporation of [³H]glucosamine and [³⁵S]sulfate into glycosaminoglycan was inhibited at day-7 and day-9, when the embryos are in the process of repair and/or recovery The cartilage specific proteglycan, an index of chondrogenesis, appeared at day-7, but the ratio of cartilagenous proteoglycan/noncartilagenous proteoglycan in the treated limbs was depressed. These results suggest that the slight retardation in chondrogenesis and/or the incomplete function of chondrocyte were induced by the treatment with HU in chick embryos. It is considered from above observation that the retardation in chondrogenesis and osteogenesis caused during the repair and/or recovery process of cell death brought about the limb deformity; shortening, thinning and bending of hind limbs.
关键词：glycosaminoglycans