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标题：CONVERSION OF LOW MOLECULAR WEIGHT RENIN INTO THE HIGH MOLECULAR WEIGHT FORM BY Cu2+ IN THE RAT KIDNEY
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作者：SHIRO MORIMOTO ; KATSUJHIKO ITO ; TAKAHIRO IWAMOTO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1984
卷号：7
期号：11
页码：820-829
DOI：10.1248/bpb1978.7.820
出版社：The Pharmaceutical Society of Japan
摘要：The low molecular weight form of renin (M.W. 40000) in the extract of rat kidneycortex was converted into the high molecular weight form (M.W. 60000) in the presence of Cu²⁺ and this conversion was abolished following pretreatment of the extract with disodium ethylenediaminetetraacetic acid. Fe³⁺, Fe(CN)₆^3-, Cd²⁺ and Zn²⁺ had no effects on the reaction of the molecular weight conversion of this enzyme. The reactivity in the formation of high molecular weight renin catalyzed by Cu²⁺ increased when the extract was previously dialyzed, indicating the presence of substance (s) that interfere with the binding reaction of renin. The high molecular weight renin produced Cu²⁺ was stable against dithiothreitol, suggesting that the formation of disulphide bridge (s) is not involved in the reaction of renin with the renin binding substance. We also confirmed that for formation of the high molecular weight renin, Cu²⁺ must react with both the renin enzyme and the renin binding substance. Our findings provide new evidence related to the mechanism of molecular weight conversion of renin in the rat kidney.
关键词：concanavalin A Sepharose 4B