文章基本信息

标题：HETEROGENEITY OF RAT LIVER SULFOTRANSFERASES
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作者：MICHIO MATSUI ; FUSAKO NAGAI
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1985
卷号：8
期号：12
页码：1048-1053
DOI：10.1248/bpb1978.8.1048
出版社：The Pharmaceutical Society of Japan
摘要：Sulfotransferases (STs)active on androsterone (AD), cortisol (CS) and 4-nitrophenol (NP) were separated by diethylaminoethyl-cellulose chromatography from cytosolic fractions of female rat liver and were divided into five ST fractions (peaks I-V) with different activities toward three substrates. The precipitates obtained in the 68% of saturation of ammonium sulfate were passed through a Sephadex G-100 column and purified by agarose-hexane adenosine 3', 5'-bisphosphate affinity chromatography. AD-ST isoenzyme (peak I) was purified 85-fold, had low CS-ST activity, was devoid of NP-ST activity and appeared to correspond to hydroxysteroid ST 1.Peaks II and V appeared to consist mainly of hydroxysteroid ST and arvl ST, respectively.
关键词：4-nitrophenol