文章基本信息

标题：A COMPARISON OF URIDINE DIPHOSPHATE-GLUCURONOSYL-TRANSFERASE AND OTHER DRUG METABOLIZING ENZYME ACTIVITIES BETWEEN TWO MUTANT STRAINS OF WISTAR RATS WITH A GENETIC DEFICIENCY IN BILIRUBIN OR ANDROSTERONE GLUCURONIDATION
本地全文：下载
作者：FUSAKO NAGAI ; MIE TAKAHASHI ; HIROSHI HOMMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1987
卷号：10
期号：8
页码：421-426
DOI：10.1248/bpb1978.10.421
出版社：The Pharmaceutical Society of Japan
摘要：A jaundiced rat strain was derived from a cross between Gunn and Wistar-Imamichi rats, and inbreeding was continued by forced heterozygosis with jaundice locus. These Gunn rats have black pigment on heads and a black stripe on their backs similar to Long-Evans rats. Wistar rats with low activity in androsterone (AD) glucuronidation were selected and inbred (LA Wistar rats). The levels of hepatic uridine diphosphate-glucuronosyltransferase (GT) and sulfotransferase (ST) activities as well as cytochrome P-450 contents were compared in these mutant strains. Gunn rats were devoid of bilirubin (BL) GT activity but had high AD GT activity. LA Wistar rats had very low AD GT activity but showed high BL GT activity. Native and Triton X-100-stimulated GT activities toward 2-aminophenol and 4-nitrophenol (NP) were much lower in Gunn rats than in LA Wistar rats. When N-nitrosodiethylamine was added to the incubation media, these GT activities were stimulated equally to high levels in both mutants. N-Nitrodiethylamine provided a similar stimulatory effect on NP GT activity. There were no significant differences in ST activities toward cortisol, AD and NP and cytochrome P-450 contents in the two mutant strains. These results indicate that Gunn and LA Wistar rats have a different deficiency in GT isoenzymes.
关键词：Triton X-100