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标题：ULTRASTRUCTURE OF SINGLE SMOOTH MUSCLE CELLS CONTRACTED BY CARBACHOL AND CALCIUM ION
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作者：IKUO MARUYAMA ; MAMORU KOBAYASHI ; CHIEKO YOSHIDA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1987
卷号：10
期号：8
页码：396-403
DOI：10.1248/bpb1978.10.396
出版社：The Pharmaceutical Society of Japan
摘要：The ultrastructural changes of single smooth muscle cells of guinea pig taenia coli during contraction evoked by Ca²⁺ (Ca) and carbachol (CCh) were investigated by transmission and scanning electron microscopy. Cells, prepared in the presence of a high concentration (140 mM) of KCl, without addition of Ca, exhibited smooth surfaced contour. The contraction of these cells were dose dependent upon Ca. With the increase in Ca concentration, the contraction was accompanied with formation of processes (0.15 μm thick and 1 μm long), membrane evaginations of various sizes and shapes and cores densely packed with filaments at the bottoms of all evaginations. Cells, prepared in the presence of 0.18 mM Ca and at a physiological concentration (2.7 mM) of KCl, had two populations : One had shallow invaginations of the cell membrane and the other had spinous processes. Cells of both types were responsive to CCh and the spinous processes disappeared during contraction but evagination did not appear. Differences in ultrastructural changes during contractions between single smooth muscle cells and intact tissues may be due to the fact that single cells are mechanically free from adjacent cells and connective tissues. It is also possible that membrane properties change during isolation, particularly when cells are isolated in Ca free medium.
关键词：carbachol contraction