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标题：Citrate Uptake by Isolated Rat Renal Brush Border Membrane Vesicles in Cadmium-Intoxicated Rats
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作者：Kazuhiro SATO ; Yukinori KUSAKA ; Qunwei ZHANG 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：1997
卷号：35
期号：3
页码：388-393
DOI：10.2486/indhealth.35.388
出版社：National Institute of Occupational Safety and Health
摘要：Epidemiological Studies have reported that the formation of renal stones is often observed in workers exposed to cadmium (Cd). Citrate is known to be a protective factor against renal stone formation. We previously reported that direct exposure to high-level cadmium impaired the citrate uptake by the brush border membrane vesicles (BBMV), which may mean that the formation of renal stones from exposure to Cd could be prevented. In the present study, to clarify the mechanism of Cd-induced renal stone formation, the characteristics of BBMV-induced citrate uptake were determined in Cd-intoxicated rats. Cd intoxication was induced by daily subcutaneous injections of CdCl₂ at a dose of 2mg Cd/kg body wt per day for 17 to 20 days. The BBMV were prepared by the divalent cation precipitation method. Citrate uptake was measured by the Millipore rapid membrane filtration technique. The citrate uptake per mg protein was not significantly different between the two groups. The volume of BBMV was significantly reduced (50%) in Cd-intoxicated rats compared with that of control rats. Percentages of the equilibrium value were also significantly inhibited in Cd-intoxicated rats.
关键词：Cadmium;Renal stones;Citrate;Brush border membrane vesicles