文章基本信息

标题：Direct Effect of Inorganic Mercury on Citrate Uptake by Isolated Rat Renal Brush Border Membrane Vesicles
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作者：Kazuhiro SATO ; Yukinori KUSAKA ; Qunwei ZHANG 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：1997
卷号：35
期号：4
页码：456-460
DOI：10.2486/indhealth.35.456
出版社：National Institute of Occupational Safety and Health
摘要：Occupational exposure to mercury has long been associated with renal proximal injury and an increased incidence of proteinuria, as has such exposure to cadmium. Renal citrate excretion is very important with respect to acid-base balance since the metabolism of citrate generates three bicarbonate ions. In this study, we exposed isolated rat renal brush border membrane vesicles (BBMV) to mercury (Hg²⁺) and examined their citrate uptake characteristics. BBMV were prepared by the divalent cation precipitation method. Citrate uptake was measured by the Millipore rapid membrane filtration method. The preincubation of BBMV with 0.5 and 2mM HgCl₂ for 1min significantly inhibited citrate uptake compared with that of BBMV without Hg preincubation. The analysis of the time course of citrate uptake during a 30-min preincubation of BBMV with 0.1mM Hg²⁺ also revealed a significant reduction in the uptake compared with that of the control BBMV without preincubation. These findings indicate that the preincubation of BBMV with mercury results in a time- and concentration-dependent inhibition of citrate uptake.
关键词：Mercury;Citrate;Brush border membrane vesicles (BBMV)