文章基本信息

标题：Rapid and Simple Analysis of Urinary Vanilmandelic Acid by High-performance Liquid Chromatography with Electrochemical Detection
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作者：Takeshi SASAKI ; Tatsuo OKA ; Naomi HISANAGA 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：1999
卷号：37
期号：2
页码：213-218
DOI：10.2486/indhealth.37.213
出版社：National Institute of Occupational Safety and Health
摘要：We examined an analytical method for urinary vanilmandelic acid (VMA) by high-performance liquid chromatography with electrochemical detection from the viewpoint of practical analysis and application. The sample pretreatment in our method is only the dilution of urine samples with citrate buffer. The calibration curve for VMA was linear within the range 0.2 to 20ng. The detection limit was 50pg at a signal-to-noise ratio of 3 and the coefficients of variation were 2.30-4.30%. Variations in the urinary levels of VMA, noradrenaline (NA) and adrenaline (Ad) during and after exercise were investigated for 5 males (mean±SD, 42.4±4.3 years of age). The high levels of urinary NA and Ad during 1hr exercise rapidly decreased after exercise, whereas urinary VMA increased after exercise rather than during exercise and decreased later. The correlation of the urinary levels of VMA and NA for 71 salesman (mean±SD, 40.6±11.7 years of age) in a field study was significantly positive (r=0.426, p<0.001). These results suggest that urinary VMA mostly reflects NA, but the excretion rate of VMA is slower than that of NA.
关键词：Vanilmandelic acid;Noradrenaline;Autonomic nervous system;Stress;Workload