文章基本信息

标题：Effects of Mineral Fibers on the Gene Expression of Proinflammatory Cytokines and Inducible Nitric-Oxide Synthase in Alveolar Macrophages
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作者：Yasuo MORIMOTO ; Tohru TSUDA ; Masami HIROHASHI 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：1999
卷号：37
期号：3
页码：329-334
DOI：10.2486/indhealth.37.329
出版社：National Institute of Occupational Safety and Health
摘要：To determine which parameters are useful for the risk assessment of man-made mineral fibers (MMMFs), we examined the gene expression of interleukin-1α (IL-1α), tumor necrosis factor α (TNFα), interleukin-6 (IL-6) and inducible nitric-oxide synthase (iNOS) in mineral fiber-exposed alveolar macrophages (AMs). Male Wistar rats were intratracheally exposed to saline or mineral fibers suspended in saline (2mg of crocidolite, chrysotile, alumina silicate refractory fiber (RF1) or potassium octatitanate whisker (TW)). Bronchoalveolar lavage was performed 4 weeks after the fiber-instillation, and the recovered AMs were stimulated by lipopolysaccharide for 2 or 6 hours. Expression of IL-1α, TNFα, IL-6 and iNOS from AMs was observed using reverse transcription-polymerase chain reaction (RT-PCR). The levels of IL-1α and IL-6 mRNA induced by mineral fiber exposure were greatest in AMs exposed to TW, crocidolite, chrysotile and RF1 in that order. However, both gene expression of iNOS and TNFα were not elevated in both crocidolite and TW exposure, despite their high pathological potential. These data suggested that IL-1α and IL-6 may be useful indicators for the risk assessment of MMMFs.
关键词：Man-made mineral fiber;Asbestos;Risk assessment;RT-PCR;Cytokine