文章基本信息

标题：MICROBIOLOGICAL ULTRAMICRO DETERMINATION OF PANTOTHENIC ACID, BIOTIN, AND NICOTINIC ACID WITH AMPLIFIED CULTIVATION
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作者：Hiroshi NAKAMURA ; Keijiro SAMEJIMA ; Yukiko SAITO 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1973
卷号：19
期号：2
页码：105-113
DOI：10.3177/jnsv.19.105
出版社：Center for Academic Publications Japan
摘要：A novel ultramicro microbioassay, amplified cultivation, was developed. The principle of the method holds true in microbioassays of all the sub-stances essentially required by microorganisms. Application of the method to analyses of PaA, biotin, and NiA with L. plantarum ATCC 8014 by the pulp disc method revealed an increase in sensitivity by a factor of 10 to 100 compared with the conventional methods.