文章基本信息

标题：STUDIES ON BIOLOGICAL FUNCTIONS OF 5'-METHYLTHIOADENOSINE VITAMIN B12-REPLACING EFFECT FOR OCHROMONAS MALHAMENSIS
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作者：Yoshikazu SUGIMOTO ; Saburo FUKUI
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1976
卷号：22
期号：2
页码：89-100
DOI：10.3177/jnsv.22.89
出版社：Center for Academic Publications Japan
摘要：An unidentified factor which can completely replace the vitamin B₁₂ requirement of a protozoan, Ochromonas malhamensis , was found in commercial yeast extract powder. The factor showed a positive sugar test with orcinol reagent. The presence of sulfur was demonstrated with platinic iodide reagent. The molecular weight, 297, and the chemical formula, C₁₁H₁₅O₃N₅S, were determined by mass spectrometry and elemental analysis. Infra-red and nuclear magnetic resonance studies indicated the presence of a thiomethyl group. The factor was hydrolyzed by treatment with acid into adenine and 5-methylthioribose, i.e. , the sugar constituent of 5'-methylthioadenosine. The factor was identified as 5'methylthioadenosine from the behavior exhibited in thin-layer chromatography and from microbiological tests for its vitamin B₁₂-replacing effect.