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标题：EFFECT OF VARIOUS SUGARS ON THE INDUCTION OF CHICK EMBRYONIC INTESTINAL DISACCHARIDASES IN THE ORGAN CULTURE SYSTEM
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作者：Setsuko YOSHIZAWA ; Mitsuyoshi SASAKI ; Sachiko MORIUCHI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1978
卷号：24
期号：3
页码：271-277
DOI：10.3177/jnsv.24.271
出版社：Center for Academic Publications Japan
摘要：Disaccharidases activities in 20-day-old chick embryonic intestine were induced by the addition of sucrose, maltose, fructose and glucose to the culture medium. However, maltitol, which cannot be digested by intestinal enzymes, showed no effect on the induction of disaccharidase activity. Kinetic study of the enzymes demonstrated that the maximum velocity ( V _max) and the Michaelis constant ( Km ) of sucrose induced disaccharidases activities of the explants showed changes similar to those observed in the chick of same developmental stage in vivo . Namely, V _max values of sucrase and maltase were increased. Km values of sucrase did not change, but that of maltase showed a significant decrease during development.