文章基本信息

标题：ROLE OF VITAMIN B12 AND ENZYMES RELATED TO METHYLMALONYL-CoA MUTASE IN A METHANOL-UTILIZING BACTERIUM, PROTAMINOBACTER RUBER
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作者：Shunsaku USDA ; Kazuyoshi SATO ; Shoichi SHIMIZUI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1978
卷号：24
期号：5
页码：477-489
DOI：10.3177/jnsv.24.477
出版社：Center for Academic Publications Japan
摘要：A methanol-utilizing bacterium, Protaminobacter Tuber, required cobalt ion or vitamin B₁₂ as its growth factor, which could be replaced by succinate among various additions to the cobalt-deficient medium. The presence of adenosylcobalamin (adenosyl-B₁₂)-dependent methylmalonyl-coenzyme A (CoA) mutase was demonstrated in the cellfree extracts of P. ruber. The specific activity of this mutase was not only fairly high in comparison with that reported with other organisms but also detected at a similar level throughout the cultivation period. The cell-free extracts of P. ruber grown on non-C₁ compounds as a sole carbon and energy source also had methylmalonyl-CoA mutase activity. Furthermore, the extracts of this microorganism catalyzed the reactions from propionyl-CoA to succinyl-CoA and from α-ketoglutarate to a-hydroxyglutarate.