文章基本信息

标题：DEMONSTRATION OF A NOVEL MOLECULAR SPECIES IN CHICK EMBRYO BRAIN: CELLULAR RETINOL-BINDING PROTEIN, F-TYPE
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作者：Mayumi SATO ; Masahide OMORI ; Yasutoshi MUTO 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1979
卷号：25
期号：1
页码：1-7
DOI：10.3177/jnsv.25.1
出版社：Center for Academic Publications Japan
摘要：By sucrose density gradient sedimentation analysis, a cytosol component of small molecular size which bound ³H-retinoic acid was detected in the chick embryo brain, exhibiting a peak on day 14 (7 days before hatching). In general, cellular retinol-binding protein (CRBP) and cellular retinoic acid-binding protein (CRABP) have been distin-guished by showing binding specificity for retinol or retinoic acid, respectively. However, the component found in embryo brain cytosol exhibited binding affinity for both retinol and retinoic acid, being similar in less ligand-specificity to CRBP (F) which was originally recognized in fish eye cytosol. Moreover, the component also showed a lack of binding affinity for prealbumin (PA), excluding the possibility that chicken plasma RBP was contaminated in the cytosol. These findings strongly suggest that a distinct molecular species of cellular binding protein for vitamin A exists in the brain cytosol of a developing chick embryo.
关键词：vitamin A;retinol;retinoic acid;cellular retinol-binding protein;cellular retinoic acid-binding protein;ligand specificity;onco-fetal protein