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标题：INTERACTION OF 1α, 24-DIHYDROXYVITAMIN D3 WITH THE CHICK INTESTINAL 1α, 25-DIHYDROXY VITAMIN D3 RECEPTOR SYSTEM
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作者：Fumio SHIMURA ; Sachiko MORIUCHI ; Norimasa HOSOYA 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1979
卷号：25
期号：5
页码：399-409
DOI：10.3177/jnsv.25.399
出版社：Center for Academic Publications Japan
摘要：The interaction of 1α, 24-dihydroxyvitamin D₃ with the 1α, 25-dihydroxyvitamin D₃ receptor system was studied in chick intestinal mucosa. The competitive receptor binding assay indicated that 1α, 24-dihydroxy-vitamin D₃ bound to a cytosol 1α, 25-dihydroxyvitamin D₃ receptor with a relatively high affinity compared with other vitamin D₃ analogs. The R-isomer of 1α, 24-dihydroxyvitamin D₃ revealed higher affinity for the receptor than the S-isomer. In the reconstituted cytosol-chromatin system, both 1α, 24(R)-dihydroxy-[³H]-vitamin D₃ and 1α, 24(S)-dihydroxy-[³H]-vitamin D₃ specifically associated with chromatin via a temperature-dependent process. The association of 1α, 24-dihydroxy-[³H]-vitamin D₃ with chromatin was reduced in the presence of competing unlabeled 1α, 25-dihydroxyvitamin D₃. Furthermore, the chromatin-associated 1α, 24-dihydroxy-[³H]-vitamin D₃ was dissociated by a high concentration of KCl, likewise 1α, 25-dihydroxy-[³H]-vitamin D₃. From these results, it is strongly indicated that 1α, 24-dihydroxyvitamin D₃ is recognized by cytosol 1α, 25-dihydroxyvitamin D₃ receptor as an analog of 1α, 25-dihydroxyvitamin D₃ and associates with chromatin by the same mechanism as 1α, 25-dihydroxyvitamin D₃.
关键词：1α,24-dihydroxyvitamin D3;lα,25-dihydroxyvitamin D3;1α,25-dihydroxyvitamin D3 receptor system;cytosol receptor;recon-stituted cytosol-chromatin;chick intestinal mucosa