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标题：PURIFICATION AND CHARACTERIZATION OF CALCIUMBINDING PROTEIN CONTAINING γ-CARBOXYGLUTAMIC ACID FROM RAT BONE
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作者：Yoko OTAWARA ; Norimasa HOSOYA ; Hisataka KASAI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1980
卷号：26
期号：3
页码：209-219
DOI：10.3177/jnsv.26.209
出版社：Center for Academic Publications Japan
摘要：A γ-carboxyglutamic acid (Gla)-containing protein was purified from rat femur cortical bone. The presence of 4 Gla residues in the rat protein was shown by amino acid analysis on alkaline and acid hydrolysates. The protein was extracted from rat cortical bone with 0.5 M EDTA (pH 7.6) and purified from the EDTA extracts by gel filtration on Sephadex G-100 and ion-exchange chromatography on DEAE-Sephadex A-25. The protein has a molecular weight of about 6, 000 on the basis of amino acid composition. The protein had 56 amino acid residues containing significant amounts of Asp, Glu, and Gla (acidic amino acids). The protein showed calcium-binding activity with K _d=0.2 mM and calcium-dependent electrophoretic mobility.
关键词：γ-carboxyglutamic acid;γ-carboxyglutamic acid-containing protein;rat cortical bone;calcium-binding activity